Morphological Population Balance Model for Studying the Effects of Seed Loading and Cooling Rates on Crystal Size and Shape Distribution in Protein Crystallisation
نویسندگان
چکیده
A morphological population balance model is applied to crystallisation of hen-egg white lysozyme (HEWL) for investigating the effects of seed loading and cooling rate on supersaturation, crystal size and shape distribution. Growth rates of individual faces and final crystal size and shape distribution were examined under varied seeding and cooling conditions. It was found that for growth only crystallisation, desired crystal size and shape can be obtained by coordinative manipulation of the seed loading and cooling rate: low seed loading and high cooling rate lead to large crystals of low aspect ratio, but care has to be taken to avoid nucleation and major shape change such as width becoming larger than the length. The interesting results not only demonstrate the effectiveness of morphological population balance simulation for protein crystallisation but also provide useful knowledge for process optimization and control.
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